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Immunocapture PCR Detection of ToLCNDV from Plant Extract by using Heterologous Virus Coat Protein Antisera

Immunocapture PCR Detection of ToLCNDV from Plant Extract by using Heterologous Virus Coat Protein Antisera

Zafar Iqbal1* and Muhammad Khurshid2 

1Akhuwat-Faisalabad Institute of Research, Science and Technology, Faisalabad, Pakistan
2Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan

*        Corresponding author: zafariqbal2009@gmail.com

Fig 1

PCR detection of immunocaptured ToLCNDV from the extracts of N. Benthamiana plants. The PCR products run on the gel were negative control (-ve), positive control (+ve) of PCR contain genomic DNA isolated from ToLCNDV inoculated plants as a template, ACMV-CP immunocaptured (1-4), TYLCV-CP immunocaptured (1-4) and PCR from genomic DNA of infected plants (1-4). 1kb DNA ladder (Thermoscientific fisher, SM#0313) was run on the gel to assess the size of required amplicon.

Fig 2

The 3-D models of all three CPs predicted by thread based I-TASSER server. Different views of ACMV-CP models are designated as A1-A3, ToLCNDV-CP (To1-To3) and TYLCV-CP (Ty1-Ty3). In each model α-helices are represented by yellow color and β-sheats by purple color.

Pakistan Journal of Zoology (Associated Journals)

April

Vol. 49, Iss. 2, Pages 425-759

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