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Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq

Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq

Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*

1Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
2Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
3College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
4Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
5College of Animal Science and Technology, China Agricultural University, Beijing 100083, China
6Tibet Academy of Agriculture and Animal Husbandry Science, Lasa 850001, China
7Nagqu grassland station, Naqu 852000, China
8Sichuan Academy of Grassland Sciences, Chengdu, Sichuan 611731, China

Guang-Xin E and Yong-Ju Zhao contributed equally in this article.

*      Corresponding author: H67738337@swu.edu.cn

 

Fig. 1.

Differential expressed gene distribution of gene ontology (GO) categories (level 2) of dominant and indominant follicles mixed pools for goat. GO functional annotations are summarized in three main categories: biological process, cellular componentand molecular function.

Fig. 2.

Top20 enriched pathway of differential expressed coding gene between dominant and indominant follicles in goat.

Fig. 3.

Candidate transcripts expression pattern of dominant and indominant follicles in goat using q-PCR.

Fig. 4.

Real time PCR validation of differentially expressed genes and long nocoding RNA in dominant and indominant follicles. Abundance of target genes was normalized relative to abundance of GAPDH gene. Bars in each panel represent the mean ± standard error (sample number = 3 and 3 parallel repetition per sample), * P < 0.05; ** P < 0.01; *** P < 0.001. BMPR1B, IGFBP5, PTGS-2, BMP2, IGFBP5, BMPR1B, LOC102181730, LOC102184274, LOC102183322 were significant up-regulated expressed in NF in compare with DF; BAX, BCL2, INHA, INSIG were significant up-regulated expressed in DF in compare with NF, which identified from RNA-seq.

Pakistan Journal of Zoology

December

Vol. 49, Iss. 6, Pages 1937-2341

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