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Identification and Genetic Evolution Analysis of One Strain of H3N2 Canine Influenza Virus Isolated from Nanjing, China

Identification and Genetic Evolution Analysis of One Strain of H3N2 Canine Influenza Virus Isolated from Nanjing, China

Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*

1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
2Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam-70060, Pakistan

*      Corresponding author: liuyongjie@njau.edu.cn

 

ABSTRACT

Nasopharyngeal swabs were collected from 6 dogs with severe respiratory syndrome at the Animal Clinics of Nanjing Agricultural University in 2014. One viral strain was isolated from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
 

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Pakistan Journal of Zoology

June

Vol. 50, Iss. 3, Pages 799-1198

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