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High Occurrence Rate of Multidrug-Resistant ESBL-Producing E. coli Recovered from Table Eggs in District Peshawar, Pakistan

High Occurrence Rate of Multidrug-Resistant ESBL-Producing E. coli Recovered from Table Eggs in District Peshawar, Pakistan

Tasbihullah1, Sadeeq ur Rahman2,*, Tariq Ali3 , Umer Saddique1, Shakoor Ahmad1, Muhammad Shafiq4, Sultan Ayaz2, Hamayun Khan1, Ijaz Ahmad1 Asadullah2, Raheela Taj5, Hafiz Nidaullah6 and Alamgir Khan7

1Department of Animal Health, The University of Agriculture, Peshawar, Pakistan
2College of Veterinary Sciences and Animal Husbandry, Section Microbiology, Abdul Wali Khan University, Mardan, Pakistan
3Center of Microbiology and Biotechnology, Veterinary Research Institute, Peshawar, Pakistan
4Collge of Veterinary Medicine, Nanjing Agriculture University, Nanjing, China
5Institute of Chemical Sciences, The University of Peshawar, Peshawar, Pakistan
6Food Technology (Food Microbiology Section), School of Industrial Technology, Universiti Sains, Malaysia
7District Head Quarter Hospital, Timergara, District Dir Lower, Pakistan
 
Tasbihullah and Sadeeq ur Rahman have contributed equally as first co-authors.

*      Corresponding author: sadeeq@awkum.edu.pk

ABSTRACT

Whilst food-producing-animals could be a pool of resistance-conferring elements in the existing animal production system in Pakistan, the issue has not yet judiciously been highlighted. This study was conducted to determine incidence of extended spectrum β lactamase (ESBL) - producing Escherichia coli in table eggs and human with history of close association with table eggs. For this purpose, a total of 200 table eggs and 50 stool samples from human were analyzed. Results showed that out of 80 E. coli isolates recovered from eggs, 20 (25%) were found to be ESBL-producers, while, of the 17 human-isolates, 4 (23.5%) were ESBL producers. PCR revealed that blaCTXM (blaCTXM-1=15 and blaCTXM-9=4) was carried by all 22 (91.6%) ESBL-producers with additional blaSHV2 (n=12) and blaNDM-1 (n=3), but no blaTEM was identified. A predominant combination of blaCTXM + blaSHV2 (n=12) followed by blaCTXM + blaNDM-1 (n=3) was determined. All these isolates (phylogroup D=14/24, A= 6/24 and B2=4/24) were found to be multidrug resistant displaying resistance against at least three different classes of antibiotics. Class 1 integron was carried by (21/24) followed by additional class 2 integron (15/24). A total of 8 isolates were harboring insertion sequence common region 1 (ISCR1), which was found to be linked with blaCTXM in 50% (4/8) isolates. Results of the current study indicate contamination of eggs with ESBL-producing E. coli suggesting to improve hygienic process for end consumer during- egg production.
 

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Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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