Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

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Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

**Faiz Muhammad^1,*, Syed Khurram Fareed¹, Urooj Zafar¹, Taseer Ahmed Khan² and Aqeel Ahmad¹**

Author Affiliations

¹Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan

²Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan

* Corresponding author: aahmad57@gmail.com

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Fig. 1.

Pictorial illustration of Tetra PCR mechanism.

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Fig. 2.

Lane 1, M. gallisepticum; Lane 2, M. synoviae; Lane 3, both MG and MS; Lane 4, M. gallinarium; Lane 5, Acholeplasma laidlawii; Lane 6, M. bovis (ATCC 25230); Lane 7, M. colloumbinsale (field isolate); Lane 8, Lactobacillus spp.; Lane 9, E. coli; Lane 10, negative control.

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December 2017

Vol. 49, Iss. 6, Pages 1937-2341

Pakistan Journal of Zoology

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Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

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Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

**Faiz Muhammad^1,*, Syed Khurram Fareed¹, Urooj Zafar¹, Taseer Ahmed Khan² and Aqeel Ahmad¹**

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Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

Development and Evaluation of Culture-Enhanced Tetra-PCR for Differential Diagnosis of Mycoplasma gallisepticum and M. synoviae

Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1

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**Faiz Muhammad^1,*, Syed Khurram Fareed¹, Urooj Zafar¹, Taseer Ahmed Khan² and Aqeel Ahmad¹**