Genomic Analysis of Glutathione S-transferases (GST) Family in Common Carp: Identification, Phylogeny and Expression
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
Sequence alignment and gene structure of common carp GST genes. The sequence alignment of carp GST genes was conducted by ClustalO. Sketch map of GST genes structure was conducted on GSDS.
Phylogenetic tree of GST gene family. Neighbor-joining-based phylogenetic tree of GST protein sequences. Including several kinds of typical vertebrates: human (Hs), chicken (Gg), frog (Xt), zebrafish (Dr), pufferfish (Tr), medaka (Ol), stickleback (Ga) and common carp (Cc). GST superfamily is labeled by three subfamilies: cytosolic GST, mitochondrial GST and MAPEG.
Analysis of conserved synteny blocks harboring GSTK genes in several vertebrates. Horizontal lines denote orthologous relationships. Abbreviations: PHC: Polyhomeotic homolog; STYK1: Serine/threonine/tyrosine kinase 1; GSTK1: Glutathione S-transferase kappa 1; MBOAT7: Membrane bound O-acyltransferase domain containing 7; TMC: Transmembrane channel-like; LMTK3: Lemur tyrosine kinase 3; PRPF31: Pre-mRNA processing factor 31 homolog; CLCN1: Chloride channel, voltage-sensitive 1; CASP2: Caspase 2, apoptosis-related cysteine peptidase.
Analysis of conserved synteny blocks harboring MGST2 genes in several vertebrates. Horizontal lines denote orthologous relationships. Abbreviations: NAA15: N(alpha)-acetyltransferase 15, NatA auxiliary subunit; RAB33B: Member RAS oncogene family; SETD7: SET domain containing (lysine methyltransferase) 7; MGST2: Microsomal glutathione S-transferase 2; ANXA5: Annexin A5; FGFBP1: Fibroblast growth factor binding protein 1; FGFBP2: Fibroblast growth factor binding protein 2; PROM1: Prominin 1; TAPT1: Transmembrane anterior posterior transformation 1.
RT-PCR based expression analysis of common carp glutathione S-transferase genes. The amplification of β-actin was used as an internal control.