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Genetic Characterization of Highly Pathogenic Avian Influenza Virus (H5N8) in Backyard Poultry Production Sector during Mid-2017 to Autumn 2018

Genetic Characterization of Highly Pathogenic Avian Influenza Virus (H5N8) in Backyard Poultry Production Sector during Mid-2017 to Autumn 2018

Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan 

Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Giza, Egypt.

wesammady83@hotmail.com 

ABSTRACT

Since incursion of highly pathogenic avian influenza virus (HPAIV) H5N8 in November 2016 in Egypt, it caused severe economic losses for both the commercial and backyard poultry production sectors. The aim of this work is to study the Situation and molecular characterization of HPAIV (H5N8) in backyard poultry production sector in Egypt. In this study, a total of 7505 samples of tracheal swabs representing 1180 backyard poultry flocks were collected based on the surveillance conducted by the GOVS (General Organization for Veterinary Services), Egypt during the period from May 2017 to August 2018. 11 positive cases were confirmed in seven governorates with a prevalence rate of 0.93% and the positive samples were mainly located in Upper Egypt. The sequence of partial HA gene was performed for 10 positive samples and they were genetically characterized as HPAIV H5N8 belongs to clade 2.3.4.4b. Except three isolates in 2018 belongs to clade 2.3.4.4A, the phylogenetic analysis revealed that our sequenced viruses were clustered together in group B Russian like reassortant H5N8 viruses of clade 2.3.4.4.  

 

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Hosts and Viruses

June

Vol.6, Iss.3, Pages 42-84

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