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Genetic Characterization of Avian Rotavirus Groups F and G in Brazilian Farms

Genetic Characterization of Avian Rotavirus Groups F and G in Brazilian Farms

Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*

Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo. Av. Professor Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, SP, Brazil.

ABSTRACT

Rotaviruses are one the causes of enteritis and diarrhea in avian species, resulting in negative impact on egg and poultry production. The objective of this work was to detect and characterize Rotavirus F (RVF) and G (RVG) found in Brazilian commercial avian farms. A total of 97 intestinal contents was collected between 2008 and 2015 from 10 Brazilian states, and submitted to two different RT-PCR, targeting a fragment of viral VP6-coding nucleotide sequence of each viral group. Amplicons were further submitted to genetic sequencing and analyzed by phylogenetic analysis. There were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
 

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Hosts and Viruses

August

Vol. 7, Iss. 4, Pages 59-108

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