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Ganoderma lucidum as a Biocontrol agent for Management of Alternaria solani, A Pathogen of Early Blight of Tomato

Ganoderma lucidum as a Biocontrol agent for Management of Alternaria solani, A Pathogen of Early Blight of Tomato

Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3

1Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; 2Center of Excellence and Molecular Biology, University of the Punjab, Lahore, Pakistan; 3School of Biological Sciences, University of the Punjab Lahore, Pakistan.

 
*Correspondence | Muhammad Asif, Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; Email: ali_asif514@yahoo.com

Figure 1:

A) Purigied mycelial growth, B) Microphotograph of conidial strutures of Alternatia solani on PDA at 100x (Labomed CXL, Labo America inc.)

Figure 2:

A) Basidiocatp, B) Mycelial growth, C) Hmenium, D) Basidiospores of G. lucidum.

Figure 3:

Antagonistic effect of G. lucidum against A. solani; A) Control (A. solani) B anc C) Dual culture (G. lucidum in white colony and A. solani in black colony showing growth zone of inhibition).

Figure 4:

Activity assay of crude protein extract aginst A. solani. Different alphabet letters indicate significant difference at 0.05 probability level.

Figure 5:

Antigungal potential of crude protein extract of G. lucidum agaist A. sonani. A) Control, B) 0.5mg/ml, C)1mg/ml, D) 2mg/ml.

Figure 6:

MIC of crude protein against A. solani by using Microspectropotmetry. Different alphabet letters indicate significant difference at 0.05 probability level.

Equation 1
Equation 2

Sarhad Journal of Agriculture

June

Vol. 38, Iss. 2, Pages 388-758

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