Annual catch and effort data were used to estimate the maximum sustainable yield (MSY) of Portunus spp. to evaluate the population state of crab fishery from Pakistani waters. The catch and effort data of crab fishery from 1999-2009 were obtained from and handbook of Fisheries Statistics of Pakistan. Two computer software programs CEDA and ASPIC were used which were based on surplus production models. From CEDA Fox, Schaeder and Pella-Tomlinson were used with initial proportion (IP) 0.9 were used because the starting catch was 90% of the maximum catch the MSY estimated value from Fox with three error assumptions (normal, lognormal and gamma) were 3378 (R2=0.590), 3360 (R2=0.582), 3369 (R2 =0.586), respectively, whereas the obtained values from Schaefer and Pella-Tomlinson with three error assumptions were 2878 (R2=0.587), 3035 (R2=0.578) and gamma were minimization failure (MF) in both models. The MSY estimated value from ASPIC from Fox and logistic model were 3652 (R2=0.8) and from logistic model were 2962 (R2=0.799), respectively. The present estimated values from surplus production models is lower than annual catch it shows that the stock of crab fishery from Pakistani waters is in overexploitation state, we may suggest to reduce the fishing efforts to sustain the crab stock from Pakistani waters for future generation.