Join Us  |   Site Map
Submit or Track your Manuscript LOG-IN

Extraction, Purification and Anti-Hyperlipidemic Activities of Total Flavonoids from Corn Silk

Extraction, Purification and Anti-Hyperlipidemic Activities of Total Flavonoids from Corn Silk

Jiandong Wu, Miao Ye and Zaigui Wang*

College of Life Sciences, Anhui Agriculture University, Anhui Hefei 230036, P.R. China 

Jiandong Wu and Miao Ye contributed equally.

*      Corresponding author:


In this study, some important parameters were optimized for effective extraction and purification of total flavonoids from corn silk, and the anti-hyperlipidemic activities was analyzed in rabbits by feeding experiment. Firstly, the high extraction yield (5.32 mg·g-1) of total flavonoids were obtained under the optimal extraction conditions, which are 80°C for 3 h with corn silk over 60% ethanol ratio of 1:30. Then, the AB-8 resin was selected for the purification of flavonoids due to the higher static adsorption and adsorption capacity than other resins. The optimum conditions for purifying flavonoids as follows: for adsorption, sample concentration was 2.542 mg·mL-1, pH was 3.7, processing volume was 4 BV; flow rate was 2 BV·h-1; temperature was 25°C. For desorption, elution solvent ethanol-water (95%, v/v) solution was 4.5 BV; flow rate was 2.0 BV·h-1. Flavonoids with content of 68.3% were obtained with a flavonoid recovery of 93.7%. The results showed that AB-8 resin revealed a good ability to purify total flavonoids. Lastly, feeding experiments results indicated that flavonoids had significant anti-hyperlipidemic activities to rabbits by reducing the content of serum total cholesterol (TC), triglycerides (TG), and low-density lipoprotein-cholesterol (LDL-C) and increasing the concentration of high-density lipoprotein-cholesterol (HDL-C).

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology (Associated Journals)


Vol. 49, Iss. 5, Pages 1937-2341


Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits

Subscribe Unsubscribe

Commons Attribution License

This license permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

Creative Commons License
Follow ResearchersLinks