Expression and Polymorphism Analysis of CFL2 Gene in Chinese Dabieshan Cattle
Expression and Polymorphism Analysis of CFL2 Gene in Chinese Dabieshan Cattle
Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*
ABSTRACT
Cofilin 2 (CFL2) is essential for skeletal muscle development and maintenance through regulating the length of actin filaments. In this study, we aimed to identify common variations in CFL2 gene and investigate their effects on growth traits in Chinese Dabieshan (DBS) cattle. By DNA sequencing and (forced) PCR-RFLP methods, three polymorphisms (g.1500G>A, g.1694T>A and g.2213C>G) were identified and genotyped in our population (n=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a strong linkage (r2>0.33), a total of four different haplotypes were constructed and the frequency of the main haplotypes AAG accounted for over 61.2 % of the total individuals. Association analysis indicated that all of the three SNPs were significantly associated with growth traits in the detected population. Furthermore, real-time PCR indicated that CFL2 mRNA was varied expressed in all studied tissues. The results of our study provide evidence that polymorphisms in CFL2 gene are associated with growth traits, and CFL2 gene could be utilized as molecular markers for future assisted selection in cattle breeding program.
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