Submit or Track your Manuscript LOG-IN

Expression Patterns of BMP15 Gene in Folliculogenesis of Buffalo (Bubalus bubalis)

Expression Patterns of BMP15 Gene in Folliculogenesis of Buffalo (Bubalus bubalis)

Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*

1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530005, PR China
2Guangxi Veterinary Research Institute, Guangxi Key Laboratory of Veterinary Biotechnology, Nanning, Guangxi, 530001, China
3The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning 53001, PR China
4Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou, Guangxi 530005, PR China

Jinfeng Liu and Yanhong Cao are the co-first authors.

*      Corresponding authors: 476819435@qq.com; qyliu-gene@qq.com

 

Fig. 1.

RT-PCR results for the buffalo BMP15 gene. A: Lane M, marker III; Lane 1-2, RT-PCR product; B: Lane M1, supercoiled DNA ladder; Lane 1-2, pMD-18T-BMP15.

Fig. 2.

Buffalo BMP15 nucleotide and amino acid sequences.

Fig. 3.

Multiple alignment and analysis of deduced amino acid sequence of BMP15.
 

Fig. 4.

Neighbor-Joining phylogenetic tree based on the BMP15 protein among some species.

Fig. 5.

The protein domain of BMP15.

Fig. 6.

The secondary structure of buffalo BMP15 protein.

Fig. 7.

The tertiary structure of buffalo, bovine and homo BMP15 protein.

Fig. 8.

Agarose gel electrophoresis of PCR product of buffalo ALK6 in different tissues. 1, skin; 2, bone; 3, granular cells; 4, cumulus cells; 5, ovary; 6, testis; 7, muscle; 8, genital ridge; 9, heart; 10, liver; 11, lung; 12, kidney; 13, brain; 14, hypophysis; 15, hypothalamus; 16, water.

Fig. 9.

The expression pattern of BMP15 during buffalo folliculogenesis and embryogenesis.

Fig. 10.

BMP15 immunoreactivity in the different structures found within buffalo ovaries and testis. A, Primordial follicle in genital ridge of foetus between 60-90 days post concep tion (dpc); B, Primordial follicle in ovary of foetus; C, Primordial follicle in ovary of adult buffalo; D, primary follicle; E, secondary follicle; F, small antral follicle; G, COC of a large antral follicle; H, testis of foetus; I, testis of adult buffalo; J, negative control of testis; K, negative control of ovary. Cyst, primordial cyst; O, oocyte; GC, granulosa cells; CC, cumulus cells; MGC, mural granulosa cells. Scale bars represent 20 μm.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe