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Expression Analysis of BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 in Rams with Different Fecundity

Expression Analysis of BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 in Rams with Different Fecundity

Weihao Chen1,2, Zhilong Tian2, Lin Ma2, Shangquan Gan3, Wei Sun1,4,* and Mingxing Chu2,*

1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
3State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China
4Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China

*      Corresponding authors: mxchu@263.net; dkxmsunwei@163.com

ABSTRACT

To elucidate the tissue expression levels of BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 genes in rams with different fecundity, quantitative real-time polymerase chain reaction was used to investigate the expression level of six genes in the brain, cerebellum, hypothalamus, pituitary, testis, epididymis, vas deferens, and adrenal gland in high fecundity (Small Tail Han sheep) and low fecundity (Sunite sheep) rams. The results were as follows: BMPR1B, GDF9, Smad1, Smad5 and Smad9 were expressed in all selected tissues, but BMP15 was specifically expressed in the epididymis. Further study indicated that the expression of BMPRIB in the brain, hypothalamus, pituitary, epididymis, and adrenal gland was significantly higher in Sunite sheep than in Small Tail Han sheep (p <0.05, p <0.01); the expression of BMP15 in the epididymis was significantly higher in Sunite sheep than in Small Tail Han sheep (p <0.01); the expression of GDF9 in the cerebellum and vas deferens was significantly higher in Small Tail Han sheep than in Sunite sheep (p <0.05); the expression of GDF9 in the adrenal gland was significantly higher in Sunite sheep than in Small Tail Han sheep (p <0.01); the expression of Smad1 in the brain and adrenal gland was significantly higher in Small Tail Han sheep than in Sunite sheep (p <0.05); the expression of Smad1 in vas deferens was significantly higher in Sunite sheep than in Small Tail Han sheep (p <0.01); the expression of Smad5 in the adrenal gland was significantly higher in Small Tail Han sheep than in Sunite sheep (p <0.05); the expression of Smad9 in the brain and epididymis was significantly higher in Sunite sheep than in Small Tail Han sheep (p <0.05, p <0.01); and the expression of Smad9 in the cerebellum and hypothalamus was significantly higher in Small Tail Han sheep than in Sunite sheep (p <0.05). This is the first study to systematically analyze the BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 genes’ tissue expression pattern in rams.
 

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Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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