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Exposure of Zebrafish (Danio rerio) to Titanium Dioxide Nanoparticle Causes Paraptosis: Evaluation of Ovarian Follicle Ultrastructure

Exposure of Zebrafish (Danio rerio) to Titanium Dioxide Nanoparticle Causes Paraptosis: Evaluation of Ovarian Follicle Ultrastructure

Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1

1Department of Biology, Science and Letters Faculty, Sakarya University, Serdivan, Sakarya, Turkey
2Department of Histology and Embryology, İstanbulFaculty of Medicine, İstanbul University, İstanbul, Turkey

*      Corresponding author: cansua@sakarya.edu.tr

Fig 1

Hıstological and electron micrographic structure of ovary of control group; a,b,c, Electron micrograph of follicles; d,e,f, light micrograph of follicules. Fe, follicular epithelium. Mi, microvilli; ZR, zona radiata; O, ooplasm; M, mitochondrium; G, golgi; RER, rough endoplasmic reticulum; Po, primary oocyte; Ca, cortical alveoli; N, nucleus; No, nucleolus; CoC, cortical alveolus stage; Vo, vitellogenic oocyte; Om, mature oocyte; Yg, yolk granule. Magnification: d, x100; e, x20; f, x40.

Fig 2

Hıstological and electron micrographic structure of ovary of 1 mg/L TiO2 exposed group; a, degeneration at microvili structure and mitochondrial deterioration (star) at follicular epithelium cells; b, cytoplasmic vacuolization and unification (asterix) of cortical alveoli; c, mitochondrial deterioration (star); d, mitochondrial swelling (square) and loss of organization of cristae (arrow) at mitochondrium; e, cytoplasmic vacuolization (black arrow) at primary oocytes; f, loss of nucleolus organization at primary oocyte; g, severe cytoplasmic vacuolization and degeneration at cortical alveoli structure; h, Eosinophilic granules accumulation at cortical alveolus stage ZR, zona radiata; Mi, microvilli; Fe, follicular epitheium; T, teca cells; M, mitochondrium,;N, nucleus; No, nucleolus; Ca, cortical alveoli; Eg, eosinophilic granule. Magnification: e, f,g, x100; h x40.

Fig 4

Lıght microscopical histological and electron micrographic strucure of ovary of 4 mg/L TiO2 exposed group. A, cytoplasmic vacuolization (red arrow) at follicular epithelium and ooplasm; b, multilamellar vesicle and cytoplasmic vacuolization (star); c, mitotic catastrophe; d, altered mitochondrium morphology and loss of cristae organization; e, cytoplasmic vacuolization (star) and multilamellar vesicle; f, dispersion at ooplasm; g, degenerated vitellogenic oocyte; h, unification of cortical alveoli (asterix). ZR, zona radiata; Fe, follicular epithelium; M, mitochondrium; ML, multilamellar vesicle; RER, rough endoplasmic reticulum; Ve, vitelline envelope; Vo, vitellogenic oocyte; N, nucleus; CoC, cortical alveolus stage; Om, mature oocyte. Magnification: g, h, x20.

Fig 3

Lıght microscopic and electron micrographic structure of ovary of 2 mg/L TiO2 exposed group. A, chromatin condensation (red arrow) and cytoplasmic vacuolization (blue arrow); b, opening between zona radiata and follicular epithelium and degeneration at microvili structure; c, mitochondrial swelling (red arrow) and loss of organization of cristae (circle); d, loss of organization of cristae (circle); e, vacuolization under zona radiata (blue arrows); f, cytoplasmic vacuolization; g, unification of cortical alveoli (asterix); h, shrinking of mature oocyte. ZR, zona radiata; Fe, follicular epithelium; Mi, microvilli; M, mitochondria; G, golgi; RER, rough endoplasmic reticulum; CoC, cortical alveolus stage; Vo, vitellogenic oocyte; Om, mature oocyte. Magnification: e, f, g) x40, h) 20 x.

Pakistan Journal of Zoology

June

Vol. 51, Iss. 3, Pages 801-1202

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