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Evaluation of the Effect of Microoperation on Porcine Parthenogenetic Embryo Competence by Raman Spectroscopy

Evaluation of the Effect of Microoperation on Porcine Parthenogenetic Embryo Competence by Raman Spectroscopy

Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*

1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, No. 100 Daxue Road, Xixiangtang District, Nanning, Guangxi Zhuang Autonomous Region, 530004, China
2Guangxi High Education Laboratory for Animal Reproduction and Biotechnology, Guangxi University, No. 100 Daxue Road, Xixiangtang District, Nanning, Guangxi Zhuang Autonomous Region, 530004, China
3Guangxi Institute of Animal Sciences, Guangxi Key Laboratory of Livestock Genetic Improvement, Nanning, Guangxi Zhuang Autonomous Region, 530001, China

*      Corresponding authors: sslu@gxu.edu.cn; khlu@gxu.edu.cn

 

Fig. 1.

Development of porcine parthenogenetic embryos with the different types of microoperation. I, intact oocytes pathenogenesis; II, the puncture of zona and plasma membrane of oocytes parthenogenesis; III, the puncture of zona and plasma membrane and aspiration cytoplasm of oocytes parthenogenesis.

Fig. 2.

The shape of parthenogenetic blastocysts generated from intact oocytes and different microoperated oocytes

Fig. 3.

Raman spectra of the spent culture media from porcine parthenogenetic blastocysts by difference types of microoperation

Fig. 4.

Pairwise subtraction Raman spectra of spent culture media with difference types of microoperation.

Fig. 5.

PCA 3D scatter plots of porcine parthenogenetic blastocysts with difference types of microoperarion.

Pakistan Journal of Zoology

August

Vol. 51, Iss. 4, Pages 1203-1598

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