Evaluation of Catalase Activity in Organs of Fresh Water Fish, Ctenopharyngodon idella Fed with Vitamin E
Muhammad Shahbaz1,2, Sajid Abdullah1*, Huma Naz3*, Khalid Abbas1, Tanveer Ahmed4, Sana Mehmood1, Muhammad Adeel Hussan5
This work was performed to evaluate the catalase (CAT) activity in Ctenopharyngodon idella fed with vitamin E (VE) supplemented diet. Four different diets viz. T1 (VE-0 mg/Kg Control), T2 (VE-200 mg/Kg), T3 (VE-350 mg/Kg) and T4 (VE-500 mg/Kg) were prepared containing different levels of VE. The C. idella was fed @ 3% of body weight three times in a day for 30 days. At the end of trail fish was dissected and tissues viz. liver, muscle and gills were obtained to analyze the CAT activity. The CAT level was reduced in all treatments when compared control. CAT activity was observed higher in the gills, muscle and liver tissues of T1 diet fed C. idella as 14.68±0.90, 12.76±0.87 and 20.34±0.99 UmL-1, respectively. Comparison among diets for CAT activity showed the trend as T4<T3<T2<T1. The maximum CAT level was noted in liver than that of gills and muscles.
Novelty Statement | This research was conducted to study the impact of Vitamin E on oxidative stress enzyme (catalase) of fish. Vitamin E has proven beneficial in protecting cellular membranes against oxidation and increases the resistance to stress.
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