Submit or Track your Manuscript LOG-IN

Escherichia coli Signal Peptidases Cleave the Signal Sequence of TK0522, a Carbohydrate Esterase from Hyperthermophilic Archaeon Thermococcus kodakarensis

Escherichia coli Signal Peptidases Cleave the Signal Sequence of TK0522, a Carbohydrate Esterase from Hyperthermophilic Archaeon Thermococcus kodakarensis

Anam Tariq, Alina Gul, Majida Atta Muhammad, Samia Falak and Naeem Rashid*

School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan

*      Corresponding author: naeem.ff.sbs @pu.edu.pk; naeemrashid37@hotmail.com

 

ABSTRACT

TK0522 gene, encoding a carbohydrate esterase, from Thermococcus kodakarensis was cloned with its native signal sequence and expressed in Escherichia coli. Heterologous gene expression resulted in production of recombinant protein in the cytoplasm which secreted gradually to the extracellular culture medium. Determination of the N-terminal amino acid sequence of the recombinant protein, in the extracellular medium, revealed that the 19 amino acid signal peptide was cleaved between Ala19 and Gly20. It seems probable that the signal peptide of TK0522 can be used for secretion of other recombinant proteins produced in E. coli.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

June

Vol. 52, Iss. 3, Pages 825-1224

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe