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Enhancing Resistance Level against Mungbean Yellow Mosaic Virus by Inducing Defense Related Enzymes in Mungbean

Enhancing Resistance Level against Mungbean Yellow Mosaic Virus by Inducing Defense Related Enzymes in Mungbean

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

1Department of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan; 2Agriculture Pest Warning and Quality Control of Pesticides, Rahim Yar Khan, Pakistan; 3Pakistan Agriculture Research Council, Research and Training Station, Bahauddin Zakariya University, Multan, Pakistan.

atifnaqvi@bzu.edu.pk  

ABSTRACT

Whitefly transmitted Begomoviruses diseases have appeared as irresistible problem in different crops of Pakistan. The research was carried out to estimate the probable role of induced systemic acquired resistance in mungbean against Mungbean yellow mosaic virus (MYMV) disease. Exogenous use of Salicylic acid (SA) and Benzothiadiazole (BTH) as elicitors enhanced the resistance in mungbean plants by triggering the SA pathway. Induced resistance was assessed by evaluating the appearance of symptoms and detection of virus titter through ELISA. Different concentrations of SA and BTH were exogenously applied to activate the inherent resistance of mungbean by the production of defense associated compounds. All treatments were supportive in suppressing plant infection as compared to infected control, but the most promising outcome of treatments was observed when SA and BTH were applied at the concentration of 5mM and 150mg/L respectively. Three weeks’ analysis of treated mungbean leaves with SA and BTH exhibited peak accumulation of phenols and defense related enzymatic antioxidants. Highest enzymatic activity was observed in treated plants followed by inoculation with MYMV. Increase in resistance by the application of SA and BTH, antioxidant enzymatic activities of SOD, POD, CAT were also increased for second week. This revealed that SA and BTH can be used as a potential source for the activation of resistance for better management of MYMV by improving protection level by the induction of systemic acquired resistance. 

 

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Pakistan Journal of Agricultural Research

March

Vol. 34, Iss. 1, Pages 1-253

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