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Mosquitocidal Activity of cyt Positive Bacillus thuringiensis Isolated from Soil Samples

Mosquitocidal Activity of cyt Positive Bacillus thuringiensis Isolated from Soil Samples

Rabia Faiz and Dilara A. Bukhari*

Department of Zoology, Government College University Lahore, Lahore 

*      Corresponding author: dilaraabbas@yahoo.com

 

Fig. 1.

Gram staining of GCU Bt 4 isolate.

Fig. 2.

Endospore staining of GCU Bt 4 (a) 18 h, (b) 30 h and (c) 48 h of sporulation.

Fig. 3.

Gel electrophoresis of 469 bp fragment of cyt 2B gene of the six most toxic Bt isolates, where 1, 2, 3, 4, 5 and 6 represent 6 Bt isolates viz GCU Bt 1, 2, 3, 4, 5 and GCU Bt 6 respectively while M stands for DNA marker.

Fig. 4.

Dengrogram representing 16S rRNA gene relatedness of six most toxic B.t. isolates NBBt 1,2,3,4,5 and 6 with accession numbers KX611122, KX611120, KX611121, KY611803, KY612210 and KY611804 with already reported genes in the Genbank DNA database.

Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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