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Efficacy of Amoxicillin and or Enrofloxacin Against Mixed Infection with Escherichia coli and Salmonella enteritidis In vitro and In vivo

Efficacy of Amoxicillin and or Enrofloxacin Against Mixed Infection with Escherichia coli and Salmonella enteritidis In vitro and In vivo

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

1Pharmacology and Pyrogen Unit, Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki P.O. Box 246, Giza 12618, Egypt; 2Department of Poultry Diseases, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki P.O. Box 246, Giza 12618, Egypt; 3Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC). Nadi El-Seid Street, Dokki P.O. Box 246, Giza 12618, Egypt.

*Correspondence | Mai A Fadel, Pharmacology and Pyrogen Unit, Biochemistry, Toxicology, Feed Deficiency Department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki P.O. Box 246, Giza 12618, Egypt; Email: dr.mai87@yahoo.com 

ABSTRACT

The present research investigated the efficacy of amoxicillin and enrofloxacin when being used alone, in a half-dose combination, and in a full-dose combination against Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was infected with the mixed infection and treated with enrofloxacin 10 mg/kg b.wt. Group five was infected and treated with a full treatment dose of amoxicillin and enrofloxacin. Group six was infected and treated with a half-dose combination of amoxicillin and enrofloxacin. Group seven was non-infected and treated with a full-dose combination of amoxicillin and enrofloxacin. Antimicrobial sensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.

Keywords | Amoxicillin, Enrofloxacin, E. coli, Salmonella, Mixed infection. 

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Advances in Animal and Veterinary Sciences

1

Vol. 11, Iss. 1, Pages 1-188

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