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Efficacy of Ultrasound Microbubble-HGF Complex for Treating Hepatic Fibrosis and its Relation with Perfusion Weighted Imaging

Efficacy of Ultrasound Microbubble-HGF Complex for Treating Hepatic Fibrosis and its Relation with Perfusion Weighted Imaging

Shou-Hong Zhang1*, Ying Zhang1, Kun-Ming Wen2, Wei Wu3 and Wen-Yan Li3

1Department of Nuclear Medicine, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang, China
2Department of Gastrointestinal Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou, China
3Institute of Ultrasound Imaging, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 404100, China

*      Corresponding author: 874645@zju.edu.cn

 

Fig. 1.

The construction of HGF expression vector and preparation of Bio-MB+Bio-CNLP. The HGF cDNAs were generated by PCR amplification and the expected ~2200 bp bands are shown (A), After transfection, the HGF expression was examined (B), in HSC-T6 by IF-IC andin L02 by Western blot (C). As a negative control, L02 cells transfected with blank pCDH vector had no detectable HGF bands.

Fig. 2.

Hydroxyproline Content.

Fig. 3.

 a. Hematoxylin/Eosin staining of liver tissue. A) stage 1 of liver fibrosis; B) stage 2 of liver fibrosis; C) stage 3 of liver fibrosis; D) stage 4 of liver fibrosis; b. Masson’s trichrome staining of hepatic tissues. A) stage 1 of liver fibrosis; B) stage 2 of liver fibrosis; C) stage 3 of liver fibrosis; D) stage 4 of liver fibrosis.

Fig. 4.

The liver of rats underwent bile duct ligation (BDL) were examined by MR-PWI.

Fig. 5.

Time-signal intensity curve (TIC) of hepatic fibrosis. a. TIC analysis of S0 stage hepatic fibrosis. The red line represents the perfusion curve of the abdominal aorta; the green line represents perfusion curve of the portal vein; and the blue line represents perfusion curve of the liver parenchyma. b. TIC analysis of S4 stage hepatic fibrosis. The red line represents the perfusion curve of the abdominal aorta; the blue line represents perfusion curve of the portal vein; and the green line represents perfusion curve of the liver parenchyma.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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