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Effects of Follicular Fluid on Developmental Competence and Gene Expression of in vitro Fertilized Sheep Embryos

Effects of Follicular Fluid on Developmental Competence and Gene Expression of in vitro Fertilized Sheep Embryos

Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5

1Department of Biology, College of Science, Aljouf University, Sakaka, Al Jouf, Saudi Arabia
2Basic Sciences Department, College of Education, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
3Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia
4Deptartement of Genetic Engineering, Faculty of Biological-Oriented Science and Technology, Kinki University, Wakayama, Japan
5Chair for DNA Research,Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia

*      Corresponding author: mogermoush@ju.edu.sa

 

Fig. 1.

The different stages of embryonic development of sheep embryos produced in vitro. A, Matured oocyte (the arrow = 1st polar body); B, Zygote (the arrow = 2nd polar body); C, 2 cells stage; D, 4 cells stage, € 8 cells stage; F, 16 cells stage; G, Morula stage; H, early blastocyst I, expanded blastocyst; J, hatching blastocyst; K, hatched blastocyst; L, blastocyst on day 7 stained with Hoechst.

Fig. 2.

Effect of sheep follicular fluid on hatching rate of blastocyst. Data are expressed as Mean ± SEM. Different superscript (a, b, c) within columns are significantly different, (P < 0.05).

Fig. 3.

Effect of sheep follicular fluid on BAX (A), BCL-2 gene expression(B) and BAX/BCL-2 (C) ratio of sheep embryos. Data are expressed as Mean ± SEM. Different superscript (a, b, c) within columns are significantly different, (P < 0.05).

Fig. 4.

Effect of sheep follicular fluid on HSBP1gene expression of sheep embryos. Data are expressed as Mean ± SEM. Different superscript (a, b, c) within columns are significantly different, (P < 0.05).

Fig. 5.

Effect of sheep follicular fluid on FGFBP-4 (A), IL-6 (B) and LAMA1 (C) gene expression of sheep embryos. Data are expressed as Mean ± SEM. Different superscript (a, b, c) within columns are significantly different, (P < 0.05).

Pakistan Journal of Zoology

August

Vol. 50, Iss. 4, Pages 1199-1600

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