Submit or Track your Manuscript LOG-IN

Effects of Different Equilibration Times on Post-Thaw Cryopreserved Semen Quality of Cattle and Buffalo Bulls

Effects of Different Equilibration Times on Post-Thaw Cryopreserved Semen Quality of Cattle and Buffalo Bulls

Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5Hamayun Khan1 and Shabana Naz6*

 

1Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan 
2Department of Livestock, Breeding and Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar
3Department of Animal Nutrition, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar 
4College of Veterinary and Animal Husbandry, Abdul Wali Khan University, Mardan 
5Department of Animal Sciences, Shaheed Benazir Bhutto University, Sheringal, Upper Dir, Pakistan

* Corresponding author: shabanapervez.2000@gmail.com

ABSTRACT

Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe