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Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3

1International Islamic University, Islamabad, Pakistan; 2Bio Resources Conservation Institute, National Agriculture Research Center, Islamabad, Pakistan; 3National Institute of Genomics and Advanced Bio-Technology, National Agriculture Research Center, Islamabad, Pakistan.

*Correspondence | Arifa Khan, International Islamic University, Islamabad, Pakistan; Email:


The purpose of the experiment was to find out the impact of various combinations of growth regulators 6-Benzylaminopurine (BAP cytokinins family) and Indole-3-acetic acid (IAA auxin family) on growth and root induction of banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L-1, respectively. Alike, Wiallium-8818 hybrid produced the longest shoot (12.20 cm) with more leaves (6.23) at 1 mg L-1 of each BAP and IAA. But Pisang variety produced maximum fresh weight (15.45 g) with a greater number of roots at 5 + 1 mg L-1 of BAP + IAA, respectively.


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Pakistan Journal of Zoology


Vol. 53, Iss. 2, Pages 401-800


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