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Effects of Epidermal Growth Factor on ASCT2 Expression in IPEC-J2 Cells Challenged by Lipopolysaccharide

Effects of Epidermal Growth Factor on ASCT2 Expression in IPEC-J2 Cells Challenged by Lipopolysaccharide

Xiaopeng Tang1*, Kangning Xiong1 and Rejun Fang2*

1State Key Laboratory Cultivation for Karst Mountain Ecology Environment of Guizhou Province, School of Karst Science, Guizhou Normal University, Guiyang, China
2Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
 
* Corresponding author: tangxiaopeng110@126.com, fangrj63@126.com

ABSTRACT

The aim of this study was to investigate the effect of epidermal growth factor (EGF) on ASCT2 expression in IPEC-J2 cells challenged by lipopolysaccharide (LPS). Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h. The gene expression of glutamine transport protein Na+-dependent neutral amino acid transporter (ASCT2) and protein expression of ASCT2 were measured. The results showed that LPS significantly (P<0.05) decreased the gene and protein expression of ASCT2 in IPEC-J2 cells. EGF significantly (P<0.05) promoted the gene and protein expression of ASCT2. EGF plus LPS group had a higher (P < 0.05) gene and protein expression of ASCT2 than that LPS treated group, although significantly (P < 0.05) lower than EGF treated group, but had no difference between the Control group. It can speculate that EGF promoted the absorption of glutamine through activating ASCT2 expression in injured intestinal epithelial cells.

 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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