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Effects of Energy Levels on Autophagy, Adipogenic Differentiation and Lipid Metabolism in Subcutaneous and Visceral Pre-Adipocytes

Effects of Energy Levels on Autophagy, Adipogenic Differentiation and Lipid Metabolism in Subcutaneous and Visceral Pre-Adipocytes

Jie Chen, Qinghua Qiu, Yanjiao Li, Xianghui Zhao, Lanjiao Xu, Xiaowen Xiong, Qingqi Wen, Mingren Qu and Kehui Ouyang*

Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Animal Nutrition and Feed Safety Innovation Team, Institute of South Grassland Research, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, China
Jie Chen and Qinghua Qiu contributed equally to this work as first authors.
* Corresponding author:


This study aimed to investigate the differences in energy levels on autophagy, adipogenic differentiation and fat metabolism between subcutaneous and visceral pre-adipocytes. C57BL/6J male mouse primary subcutaneous and visceral pre-adipocytes were isolated and in-vitro adipogenic differentiation was performed in high-glucose medium (4500 mg/L glucose, HGM) or low-glucose medium (1000 mg/L glucose, LGM). Lipid contents in adipocytes were determined by oil red-O staining. Autophagy, adipogenic differentiation and fat metabolism were evaluated by western blot. Subcutaneous pre-adipocytes proliferation rate, differentiation degree and lipid accumulation were higher than that of visceral pre-adipocytes both in HGM and LGM. In HGM group, autophagy marker genes i.e., microtubule-associated protein 1A/1B-light chain 3 (LC-3) and lipolysis protein i.e., hormone-sensitive lipase (HSL) expression in visceral pre-adipocytes were higher than that in subcutaneous pre-adipocytes, but adipogenesis transcriptional factors such as sterol regulatory element-binding protein 1c (SREBP-1c) and peroxisome proliferator-activated receptor-gamma (PPAR-γ) were lower than that in subcutaneous pre-adipocytes. Moreover, the LGM group increased the expressions of LC-3, reduced the p62 in both subcutaneous and visceral pre-adipocytes, and decreased the expression of the PPAR-γ, SREBP-1c, and HSL. It is shown that triglyceride accumulation of pre-adipocytes in LGM was more than that of HGM. There were differences in autophagy, adipogenesis differentiation and lipolysis between subcutaneous and visceral pre-adipocytes in vitro. In addition, the low energy levels on the adipogenesis differentiation and lipid metabolism of subcutaneous pre-adipocytes were greater than that of visceral pre-adipocytes, but not the responses to autophagy.

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Pakistan Journal of Zoology


Vol. 55, Iss. 2, Pages 501-1002


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