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Effects of Carbon Sources and Growth Regulators on the Tissue Culture of Sugarcane

Effects of Carbon Sources and Growth Regulators on the Tissue Culture of Sugarcane

Mazhar Ullah and Mohammad Sayyar Khan*

Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar 25000 Khyber Pakhtunkhwa, Pakistan.

 
*Correspondence | Mohammad Sayyar Khan, Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar 25000 Khyber Pakhtunkhwa, Pakistan; Email: sayyarkhankazi@aup.edu.pk

Figure 1:

Callus induction in CP 77/400 on CM with sucrose as carbon source. A= Control, B and C= Callus induction after 10 days and D-F= callus after 3 subcultures.

Figure 2:

Callus induction in CP 77/400 on CM with glucose as carbon source. A=Control, B and C= Callus induction after 10 days and D-F= callus after 3 subcultures.

Figure 3:

Callus induction in CP 77/400 on CM with fructose as carbon source. A= Control, B and C= Callus induction after 10 days and D-F= callus after 3 subcultures.

Figure 4:

Shoot induction in CP 77/400 after 35 days on SM-2 using sucrose as carbon source. A and B= shoot induction on SM-2 and C= Control.

Figure 5:

Shoot induction in CP 77/400 after 35 days on SM-2 using glucose as carbon source. A and B= shoot induction on SM-2 and C= Control.

Figure 6:

Shoot induction in CP 77/400 after 35 days on SM-2 using fructose as carbon source. A and B= shoot induction on SM-2 and C= Control.

Figure 7:

Root induction and subsequent acclimatization of CP 77/400 to greenhouse condition.

Equation 1
Equation 2

Sarhad Journal of Agriculture

March

Sarhad Journal of Agriculture, Vol.40, Iss. 1, Pages 01-262

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