Probiotics are useful in modulating and strengthening the gut microbiota. Present study was designed to determine the effect of three previously characterized probiotic lactobacilli i.e. L. fermentum IKP 23, L. fermentum IKP 111 and L. salivarius IKP 333 on histomorphology of small intestine and D-xylose absorption capacity in broiler challenged with S. enteritidis. Negative control group was not supplemented with probiotics. Positive control groups received only the challenge bacteria (S. enteritidis) ATCC 13076 at day 07. Groups (3, 4 and 5) received probiotics (IKP23, IKP111, IKP333) at day 01 to 35 and challenge bacteria at day 07 in prevention model. Groups (6, 7 and 8) started receiving probiotic at day 07 to day 35 and challenge bacteria at day 07 in treatment model. Group 09 started receiving commercial probiotic Protexin (1g/litre) at day 01 to 35 and challenge bacteria at daye 07. Group 10 started receiving antibiotic at day 01 to 10 and challenge bacteria at day 07. Birds were challenged with a single dose of 106 cfu of Salmonella enteritidis by oral gavage. D-xylose absorption capacity and gut morphometric parameters (villus height, crypt depth and villus height to crypt depth ratio) were studied at day 35. Broiler administered with IKP23, IKP111 and IKP333 significantly improved villus height and villus height to crypt depth ratio as compared to positive control. D-xylose absorption was also enhanced in groups administered with probiotics. It is concluded that IKP23, IKP111 and IKP333 may improve gut morphometric parameters and absorption capacity of broiler challenged with S. enteritidis which insinuate for their possible role in efficient broiler production.