In the present study, effect of five different temperatures (24 ºC, 26 ºC, 28 ºC, 30 ºC and 32 ºC) was studies on different colony characteristic of post-hibernated queens of Bombus terrestris during flight activity period and solitary phase of development. Results showed that survival rate and colony initiation rate of the queen was highest at 28 ºC and 30 ºC. Different parameters in the initial stage of colony growth like pre-oviposition period and emergence timing of the first worker were observed best at 28 and 30 ºC. Number of workers produced in the first brood and in the total lifespan of the colony was also observed higher at 28 ºC. Higher number of progeny queens was produced at temperature treatment of 30 ºC. Therefore, 28-30 ºC temperature exposure to the post-hibernated queens was found the best for artificial rearing of B. terrestris and developing strong colony characteristics.