This study was planned to estimate the effect of different dietary lysine regimens on slaughter and carcass traits in Lakha, Mianwali, Mushki and Peshawari varieties of indigenous Aseel chicken. A total of 240 day-old chicks, 60 from each of the variety were taken randomly and sub-divided equally into three groups (A, B and C). They were offered three lysine regimens i.e., L1 constituting 1.3% lysine from 0-6th week (1-phased) to group A and L2 containing 1.4-1.2 % lysine where 1.4% lysine was offered from 0-3rd week and 1.2% lysine from 4-6th week (2-phased) to group B, while, L3 lysine regimen having 1.5%, 1.3%, 1.1% lysine was offered from 0-2nd, 3-4th and 5-6th week, respectively (3-phased) to group C. These birds were placed in Randomized Complete Block Design (RCBD) with a factorial arrangement of 3(lysine regimens) × 4 (varieties) × 20 (replicates) with one bird in each replicate under standard conditions of house management. For slaughter and carcass characteristics at the age of eighteen weeks, 72 birds including 18 from each variety with 6 from each group were randomly selected and slaughtered. The collected data were analyzed by statistical analysis system (SAS, 9.1) software through factorial Analysis of Variance technique (ANOVA) and means were compared by Duncan’s Multiple Range (DMR) test. The findings of this study revealed L3 lysine regimen to be the best for improving the slaughter characteristics including weight of head, shanks, lungs, liver, gizzard, heart, and carcass yield comprising percent weight of breast and thigh.