Diapause is the very important stage in the artificial rearing of bumblebees that entails costs that become visible after diapause completion. In the present study different diapause temperature (-2-0 ºC), (1-3 ºC), (4-6 ºC) and duration (two and three months) showed the highest survival rate of the queens after diapause period of two months at 1-3 ºC. Highest colony initiation rate was observed at 1-3 ºC for three months duration. Early pre-oviposition period and early emergence of the first worker in a colony was also observed at three months diapause period at diapause temperature of 1-3 ºC. Larger colony size with the highest number of workers, males and progeny queens were also observed at 1-3 ºC for three months period which suggests the importance of diapause conditions on bumblebee colony characteristics.