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Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli

Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli

Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*

1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
2Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
3School of Basic Medical Science, Southwest Medical University, Luzhou 646000, China
4Institute of Veterinary Immunology and Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
5Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
 
Yang Yang and Hong Zhou contributed equally to this work.
 
* Corresponding author: yzgqzhu@yzu.edu.cn

ABSTRACT

The objective of this study was to investigate the effect of hcp1, a main structural gene of type VI secretion system (T6SS), on the biological characteristics related to the virulence factor of F18 Shiga toxin-producing Escherichia coli (STEC). In this study, based on the construct of the wild type F18 STEC (F18), hcp1 deletion mutant (F18ab△hcp1) and complement strain (F18ab△hcp1/phcp1), their pathogenic differences were compared, and the function of hcp1 was discussed from the aspects of growth curve, biofilm formation, adherence, invasion, cytotoxicity, and so on. The results showed that hcp1 of the T6SS did not influence bacterial growth and biofilm formation. In F18ab△hcp1, bacterial motility, adherence and invasion towards the intestinal porcine enterocyte cells (IPEC-J2) were reduced significantly, and the cytotoxicity decreased. The results of RT-PCR showed that in F18ab△hcp1, the flagella gene flic and type I fimbriae fimA were significantly down-regulated. In conclusion, hcp1 gene of F18 STEC is related to flagella expression, bacterial adherence, invasion, and secretion of Stx2e toxin. T6SS hcp1 is proved to be closely involved in the pathogenesis of F18 STEC.

 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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