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Effect of LncRNA TINCR on Hepatocellular Carcinoma Hep-3B Cell Growth and Chemosensitivity

Effect of LncRNA TINCR on Hepatocellular Carcinoma Hep-3B Cell Growth and Chemosensitivity

Guang Yin, Wencheng Kong, Yuqiang Shan, Jian Zhang, Rongchao Ying and Sixin Zheng*

Department of Gastroenterological Surgery, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China

 
* Corresponding author: drzengzhengxu@gmail.com

ABSTRACT

Present study was aimed to investigate the effect of LncRNA TINCR on the proliferation, invasion, migration and chemotherapy sensitivity of hepatocellular carcinoma Hep-3B cells and its mechanism. In present study Hep-3B cells cultured in vitro were divided into control group (normal culture), NC-siRNA group (transfected with NC-siRNA), and TINCR-siRNA group (transfected with TINCR-siRNA), In addition, cells treated with cisplatin after transfection of TINCR-siRNA were designated as the TINCR-siRNA + cisplatin group, and only cells treated with cisplatin were designated as cisplatin group. The expression levels of TINCR and miR-646 in the cells of the control group, NC-siRNA group and TINCR-siRNA group were tested by RT-PCR, and the cell proliferation, invasion and migration ability of the three groups were detected by the CCK-8 method and Transwell chamber experiment. Cell proliferation and apoptosis of NC-siRNA group, TINCR-siRNA group, cisplatin group and TINCR-siRNA+cisplatin group were checked by CCK-8 method and flow cytometry; and targeted binding relationship between TINCR and miR-646 was measured by dual luciferase reporter gene experiment. According to results of our study, compared with the control group there was no significant difference in the parameters of Hep-3B cells after transfection of NC-siRNA (P> 0.05), but the expression level of TINCR and cell proliferation, invasion and migration ability were significantly reduced, and the expression level of miR-646 in the cells was significantly increased, which were statistically significant compared with the control group or the NC-siRNA group (P<0.05). In addition, transfection of TINCR-siRNA can also enhance the antiproliferative and pro-apoptotic effect of cisplatin on Hep-3B cells. Double luciferase reporter gene experiments confirmed that TINCR can target miR-646. We concluded Down-regulating the expression of TINCR can inhibit the proliferation, invasion and migration of hepatocellular carcinoma Hep-3B cells and enhance its sensitivity to chemotherapy with cisplatin, and the mechanism may be related to the targeted regulation of miR-646 expression.

 

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Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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