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Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*

School of Life Science, FuYang Teachers College, Fuyang, Anhui 236041, China 

*      Corresponding author: qucq518@163.com

Fig 1

Morphological characteristics of ADSCs cultured for 24 h after thawing. A, control group (CK); B, GSH group (100×); C, FH group (100×); D, GSH + FH group (200×).

Fig 2

Morphological characteristics of ADSCs cultured for 24 h after thawing in which the cryopreservation solution contained H2. A, control group; B, GSH group (100×); C, FH group (100×); D, GSH + FH group (200×).

Fig 3

Values of MTT of post-thawed ADSCs treated with GSH and FH. Data are shown as mean values ± SD (n=3). *p<0.05 compared with the control. Control (CK), GSH (100 µM GSH group), FH (10 µM FH group) and GSH + FH (100 µM GSH+10 μM FH group).

Fig 4

Values of MTT of post-thawed ADSCs treated with GSH, FH and Hydrogen gas. Data are shown as mean values ± SD (n=3). *p<0.05 compared with the control. Control (CK), GSH (100 µM GSH group), FH (10µM FH group) and GSH+FH (100 µM GSH+10μM FH group).

Fig 5

Analysis of apoptosis in post-thaw cells by flow cytometry. A, conventional cryopreservation solution (control); B, cryopreservation solution containing GSH; C, cryopreservation solution containing FH; D, cryopreservation solution containing GSH and FH.

Pakistan Journal of Zoology (Associated Journals)

June

Vol. 49, Iss. 3, Pages 761-1149

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