Submit or Track your Manuscript LOG-IN

Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

Effect of Fasudil Hydrochloride and H2 on the Post-Thaw Viability of Cryopreserved Porcine Adipose-Derived Stem Cells

Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*

School of Life Science, FuYang Teachers College, Fuyang, Anhui 236041, China 

*      Corresponding author: qucq518@163.com

ABSTRACT

The present study is to explore the effect of fasudil hydrochloride and H2 on the post-thaw viability of cryopreserved porcine adipose-derived stem cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopreservation solution. After 2 months, MTT assay showed significant differences in the proportion of adherent viable cells in the FH group and GSH group compared with the control group (p < 0.05), and the FH+H group had a more beneficial effect on post-thaw survival of cryopreserved ADSCs compared with the GSH+H and GSH+FH+H groups. The use of FH and H in long-term storage has the potential to be helpful in commercial and clinical application of ADSCs.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

February

Vol. 50, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe