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Drug Susceptibility Profile of Staphylococcus aureus Isolated from Mastitic Milk of Goats and Risk Factors Associated with Goat Mastitis in Pakistan

Drug Susceptibility Profile of Staphylococcus aureus Isolated from Mastitic Milk of Goats and Risk Factors Associated with Goat Mastitis in Pakistan

Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*

1Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
2Department of Zoology, Government Postgraduate College for Women, Sub Campus, University of Gujrat, Rawalpindi, Pakistan
3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
4Yunnan Agricultural University, Kunming, China
5College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan

*      Corresponding authors: sdeeeq@awkum.edu.pk; amjad.aqib@uaf.edu.pk

 

ABSTRACT

The current study investigates the incidence of subclinical mastitis (SCM), associated risk factors, involvement and antimicrobial susceptibility of Staphylococcus aureus in the development of SCM in District Faisalabad of Pakistan. For this purpose, a total of 384 goat milk samples were screened for SCM through surf field mastitis test (SFMT) and mastitis-positives cases were further investigated for isolation of S. aureus using standard procedures. Coagulase gene was PCR amplified from the clinical isolates to categorize them into Coagulase positive Staphylococci (CPS) and coagulase negative Staphylococci (CNS). A questionnaire was used to record risk factors associated with occurrence of SCM and results were analyzed using non-probability statistical analysis. Results indicated that an overall 63.28% (243/384) of goats were found positive for SCM, of which 58.85% (143/243) revealed Staphylococcal growth among them 69.93% (100/143) were CPS, while 30.07% (43/143) were found as CNS. Drug susceptibility against penicillins, cephalosporins, macrolides and other drugs showed that 50% of CPS and 30% of CNS were found multi-drug resistant-exhibiting resistance against more than two or more than two classes of drugs. None of the CPS while 20 % from CNS isolates were 100% susceptible to all kinds of drugs tested. Finally, age, grazing system, use of beta-lactam antibiotics, parity, and poor hygiene were potential risk factors. Altogether, the study concluded high incidence of SCM and isolation rate of staphylococci that were found resistant against most of the commonly used antibiotics.
 

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Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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