Submit or Track your Manuscript LOG-IN

Distribution of Phenotypic and Genotypic Antibiotic Resistance in E. coli Isolates along the Production and Supply Chain of Pork around Hubei Province of China

Distribution of Phenotypic and Genotypic Antibiotic Resistance in E. coli Isolates along the Production and Supply Chain of Pork around Hubei Province of China

Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1

1Key Lab of Agriculture Microbiology, Huazhong Agriculture University, Hubei, China
2Department of Animal Health, The University of Agriculture, Peshawar, Pakistan
3Department of Poultry Sciences, The University of Agriculture, Peshawar, Pakistan
4Department of Livestock and dairy development, Peshawar, Pakistan
5University of Veterinary and Animal Sciences, Lahore, Pakistan
6Khyber Medical University, Peshawar, Pakistan

*      Corresponding author: sbvetdr@yahoo.com

 

ABSTRACT

Using MacConkey agar, E. coli were isolated from 285 samples including 125 tonsil swabs (4-6 weeks old healthy pigs from 5 farms), 80 tissue samples from different slaughter houses (20 each intestine, liver, meat and kidney), and 80 samples from super- and wet-markets (each 20 meat and liver) collected both in summer and winter. Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT, CIP, SXT, AMP, LIN and AZM) according to the disc diffusion method and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV} using mPCR. Resistance for LIN was the highest in overall (96.3%, 77/80) isolates as well as from pig farms (100%, 20/20) and different markets (100%, 20/20, 85%, 17/20). Resistance for other antibiotics such as AMX, TET, AMP and SXT was found 82.5% (66/80), 63.7% (51/80), 58.7% (47/80) and 50% (40/80), respectively. The most prevalent ARGs in the isolates recovered from pig farms was blaTEM (100%, 20/20), followed by blaCMY-2 (80%, 16/20), tetA and tetB (60%, 12/20) and tetC (50%, 10/20). E. coli became more and more diverse along the PSCP with group B2 being the most prevalent. Besides multiple drug resistance, they share many traits with the human pathogenic isolates based on virulence gene contents that may pose a potential threat to public health.

 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

August

Vol. 51, Iss. 4, Pages 1203-1598

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe