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Development of 17 Novel Cross-Species Microsatellite Markers for Dabry’s Sturgeon (Acipenser dabryanus) from Chinese Sturgeon (Acipenser sinensis) via Next-Generation Sequencing

Development of 17 Novel Cross-Species Microsatellite Markers for Dabry’s Sturgeon (Acipenser dabryanus) from Chinese Sturgeon (Acipenser sinensis) via Next-Generation Sequencing

Yacheng Hu1,2, Xueqing Liu1,2, Jing Yang1,2, Kan Xiao1,2, Binzhong Wang1,2 and Hejun Du1,2,*

1Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang Hubei 443100, China
2Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang Hubei 443100, China

Yacheng Hu and Xueqing Liu contributed equally to this investigation and should be considered co-first authors.

*     Corresponding author: duhejun21@126.com

 

ABSTRACT

In this study, we report 17 novel cross-species microsatellite markers for Dabry’s sturgeon via next-generation sequencing from Chinese sturgeon (Acipenser sinensis). The 17 microsatellites were polymorphic with 3 to 6 alleles per locus and the total number of alleles is 80. The mean expected heterozygosity (HE, 0.506 to 0.781), observed heterozygosity (HO, 0.542 to 1), Hardy-Weinberg departure value (d, -0.042 to 0.427), polymorphic information content (PIC, 0.482 to 0.753) and the Shannon-Wiener Diversity Indices (H’, 0.827 to 1.565) of all the 17 polymorphic loci indicated a high level of informativeness. Those markers will be useful tool to study the genetic diversity of Dabry’s sturgeon.
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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