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Development of Expressed Sequence Tag-Single Nucleotide Polymorphism Markers in Swimming Crab, Portunus trituberculatus

Development of Expressed Sequence Tag-Single Nucleotide Polymorphism Markers in Swimming Crab, Portunus trituberculatus

Shaokun Lu1,2, Ronghua Li1,2*, Chunlin Wang1,2, Changkao Mu1,2 and Weiwei Song1

1Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
2Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China
 
* Corresponding author: lironghua@nbu.edu.cn

ABSTRACT

In this study, sixteen SNP (single nucleotide polymorphism) markers were developed from EST (expressed sequence tag) database of Portunus trituberculatus. Polymorphism evaluation was tested on 30 wild individuals of P.trituberculatus collected from Xiangshan, Zhejiang province, China. The minor allele frequency ranged between 0.292 and 0.500, with an average of 0.384. The expected and observed heterozygosities (He and Ho) ranged from 0.422 to 0.545 and from 0.000 to 1.000 respectively. Four loci were found deviate significantly from Hardy-Weinberg equilibrium. Blast results give significant hits for nine confirmed SNP-associated sequences, some of these genes are associated with important immunological functions. These EST-derived SNP markers will be useful tools for fisheries management and conservation programme of P.trituberculatus.
 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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