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Determination of Atropine Residues in Beef by High Performance Liquid Chromatography

Determination of Atropine Residues in Beef by High Performance Liquid Chromatography

Shuhuan Li1*, Yongheng Bo2, Youzhi Li3 and Xiuzhen Yang2

1Department of Food Science and Engineering, Shandong Agricultural and Engineering University, Jinan 250100, China
2Shandong Livestock Product Quality and Safety Center, Jinan 250022, China
3Institute of Veterinary Drug Quality Inspection of Shandong Province, Jinan 250022, China
 
Shuhuan Li and Yongheng Bo contributed equally to this article.
 
* Corresponding author: lishuhuan418@126.com

ABSTRACT

Atropine is an anticholinergic drug from natural plants, and has been widely used in the clinical applications of animals and humans. However, in livestock production, excessive or improper use of atropine will lead to atropine residues in meat. When people eat animal meat from these sources, it will pose a potential threat to human health. Thus, in production practice, atropine residues in meat are usually determined quantitatively. In this study, high performance liquid chromatography (HPLC), a simple but potent method was used to quantify atropine residues in beef. The results indicated that the method could measure atropine residues accurately, with the average recoveries between 82.37 and 88.31 % when the additional concentration ranged from 0.5 to 5.0 mg/kg, and a detection limit of 0.25 mg/kg, providing an effective and reliable method for the detection of atropine residues in beef. 
 

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Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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