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Detection of Insecticide Residues in Honey of Apis dorsata F. from Southern Punjab, Pakistan

Detection of Insecticide Residues in Honey of Apis dorsata F. from Southern Punjab, Pakistan

Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4

1Department of Entomology, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Pakistan
2Department of Entomology, Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan
3Plant Pathology Section, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
4Department of Forestry, Range and Wildlife Management, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

*    Corresponding author: aslam_farooqi1770@yahoo.com

ABSTRACT

A simple and fast analytical method using High Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples were spiked at 0.1 and 0.01 mg/kg. The mean recoveries of these insecticides in the spiked samples were 74-92% with relative standard deviation less than 20%. The residues of imidacloprid, endosulfan and deltamethrin were detected with a range of 0.005-0.055 mg/kg while other insecticides were not detected in any samples. The results obtained, point the urgent need to establish reliable monitoring programs in honey.
 

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Pakistan Journal of Zoology (Associated Journals)

August

Vol. 49, Iss. 4, Pages 1151-1546

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