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Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers

Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers

Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8

1Department of Biology, Virtual University, Lahore, Pakistan
2Department of molecular Biology, Virtual University of Pakistan
3Department of Breeding and Genetics, Cholistan University of Veterinary & Animal Sciences, Bahawalpur
4Punjab Livestock and Dairy Development Department, Chakwal, Pakistan
5Poultry Research Institute, Rawalpindi, Pakistan
6University of Veterinary and Animal Sciences, Lahore, Pakistan
7Department of Microbiology, Friedrich Loeffler Institute, Jena, Germany
8Department of Pathology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan

*      Corresponding author: Umihabiba25@gmail.com

 

Fig. 1.

Amplification product of VP2 gene from IBDV. L, DNA marker; Lanes 3 to 8, positive samples; PC, positive control; NC, negative control.

Fig. 2.

Phylogenetic tree based on the VP2 gene sequences of IBDV. IBDV detected in present study have been represented with black circles.

Fig. 3.

VP2 protein based analysis showing amino acid pattern of IBDV. The amino acid alanine at the position 222 and other amino acids I242, I256, I294 and S299 is characteristic for vvIBDV.

Pakistan Journal of Zoology

April

Vol. 52, Iss. 2, Pages 425-824

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