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Detection and Molecular Characterization of Virulent Newcastle Disease Virus in Ducks (Anas platyrhynchos domesticus)

Detection and Molecular Characterization of Virulent Newcastle Disease Virus in Ducks (Anas platyrhynchos domesticus)

Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7

1Department of Pathobiology, KBCMA, CVAS, Sub Campus University of Veterinary And Animal Sciences, Narowal
2Department of Zoology, University of the Punjab, Lahore 
3Department of Pathology, University of Veterinary and Animal Sciences, Lahore 
4Department of Biotechnology, Baluchistan University of Information Technology, Engineering Management Science, Quetta
5Department of Zoology, University of Education, Lahore
6ADL Office,16 Cooper Road, Lahore
7IMBB Department, University of Lahore

*  Corresponding author: Bz-leo@hotmail.com

 

ABSTRACT

The present study was conducted to detect, isolate and characterize the Newcastle disease virus (NDV) in ducks. For this purpose, a total of 100 samples of cloacal and 100 oropharyngeal swabs of ducks (Anas Platyrhynchos domesticus) were collected from different villages of four tahsils of Sheikhupura during period (May to July 2017). NDV was isolated from field samples by inoculating the suspected samples into embryonated eggs of 9-11 days. Isolated virus was confirmed through haemagglutination and haemagglutination inhibition test. Virus isolation rate of NDV from cloacal swab was 12% (6/50) and from oropharygeal swab was 8% (4/50). The 238-bp region of F gene of NDV was amplified by using specific primers of reverse transcriptase-polymerase chain reaction (RT-PCR). Out of 50 field samples 11 cloacal swabs (34%), were positive and out of 50, nine oropharyngeal swabs (18%) were positive for NDV through RT-PCR. DNA sequencing was performed by amplifying the RT-PCR amplicon targeted the F gene. Phylogenetic tree was constructed. It was concluded that virulent strains were existed in ducks and more than one genotype of NDV was prevalent in ducks.
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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