Submit or Track your Manuscript LOG-IN

DNA-Based Gender Identification of Punjab Urial (Ovis vignei punjabiensis) using Non-Invasive Sampling

DNA-Based Gender Identification of Punjab Urial (Ovis vignei punjabiensis) using Non-Invasive Sampling

 Amna Arshad Bajwa1, Saher Islam1, Muhmmad Imran1, Karman Ashraf2, Arman Khan1, Muhammad Farhan Khan3, Imran Rashid2, Muhammad Yasir Zahoor1Waseem Ahmad Khan4 and Wasim Shehzad1*

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
2Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan
3Department of Forensic Sciences, University of Health Sciences, Lahore
4Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore 4942, Pakistan

*      Corresponding author: Wasim.shehzad@uvas.edu.pk

ABSTRACT

Gender identification of wild ungulates is imperative and critical, particularly for juveniles. Accurate gender determination is important for studying ecology and conservation biology. Therefore, a simple molecular technique, that is precise with non-invasive sampling approaches such as faeces, would be valuable. In the present study a set of molecular markers was developed exploiting the AMLx/y gene to assess gender of Punjab urial population in Kala Bagh, using faecal samples as the DNA source. In our study, among 92 urial samples, 54 (58.69%) were identified as female samples, 34 (36.95%) were recognized as males while for remaining 4 (4.34%) samples there was no amplification, resulting in their gender not being able to be assessed. This non-invasive sampling technique accurately identifies gender and has importance in developing conservation application for Punjab urials as well as equally applicable to other wild ungulates.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe