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Cysticidal Activity of Trachyspermum ammi Essential Oil against Acanthamoeba Isolates from Dental Clinic and Hospital Water Networks

Cysticidal Activity of Trachyspermum ammi Essential Oil against Acanthamoeba Isolates from Dental Clinic and Hospital Water Networks

Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3

1Department of Biology, Faculty of Science, University of Tabuk, P.O. Box 741, Tabuk 71491, Saudi Arabia
2Water Pollution Research Department, Environmental Research Division, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt
3Medicinal and Aromatic Plants Research Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt

*      Corresponding author: wafaahikal@gmail.com

 

ABSTRACT

Acanthamoeba genus is widespread in the environment, including the hospital water sources and medical devices especially in dental units and dialysis machines. It poses a threat to public health as it is responsible for human infection. In inappropriate conditions, it forms cyst with high levels of resistance to disinfection methods. The aim of this study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of the Acanthamoeba cysts.The results showed the cysticidal activity of T. ammi essential oil, LC50 values estimated to be 73.31, 61.69, 57.21 and 53.01 μg/mL with 24, 48, 72 and 96 h, respectively. This is the first report of in vitro cysticidal activity of T. ammi essential oil and the activity of this oil makes it a promising anti-Acanthamoeba.
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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