Join Us  |   Site Map
Submit or Track your Manuscript LOG-IN

Construction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus

Construction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus

Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*

1College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, China
2College of Life Science, Shihezi University, 832000, Shihezi, Xinjiang, China
3College of Medicine, Shihezi University, 832000, Shihezi, Xinjiang, China

*      Corresponding author: 604118228@qq.com; chuangfu_chen@163.com

ABSTRACT

Brucella is pathogenic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of apoptosis rate after exposure of RAW264.7 to omp10 mutants at 12 h, 24 h and 48 h compared to RAW264.7 exposed to 2308. Results indicate that Δomp10 mutant would affect its survival and pathogenicity.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology (Associated Journals)

October

Vol. 49, Iss. 5, Pages 1547-1936

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe

Commons Attribution License

This license permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

Creative Commons License
Follow ResearchersLinks