Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
Hafiz Muhmmad Tahir1*, Rabia Yaqoob2
ABSTRACT
In the present study, the efficiency of Taq DNA polymerase, an enzyme traditionally used in gene amplification, was compared with the newly developed amplification method, PuRe Taq Ready-To-Go PCR beads. One hundred seventy samples, including both fresh and up to five years old tissue samples, were compared. Taq DNA polymerase was found to be less efficient compared to the PuRe Taq Ready-To-Go PCR beads for amplification of the 12S rDNA gene. However, difference in the efficiency of both procedures was not statistically significant for the 16S rDNA gene. Furthermore, Taq DNA polymerase was found only efficient for fresh samples while PuRe Taq Ready-To-Go PCR beads were equally efficient for both old and new tissue samples.
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