Protective Efficacy of Combined Vaccines against Newcastle Disease and Infectious Bronchitis Viruses in Broiler Chickens in Egypt
Protective Efficacy of Combined Vaccines against Newcastle Disease and Infectious Bronchitis Viruses in Broiler Chickens in Egypt
Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1
ABSTRACT
A study has been carried out to evaluate different local and imported combined inactivated NDV+IBV vaccines and investigate its ability to protect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 days all groups were challenged with a dose of 105.5 EID50/bird of Variant 2 genotype (1494) IBV through oculo-nasal rout. Blood samples were collected randomly from 10 birds from each group at day 7, 14, 21, 28, 35 and 42 . Organs of 3 birds/each group were collected (trachea, bronchi and kidneys) for PCR at 3, 7 and 10 days post vaccination and post challenge as well. Groups 3 &4 showed high protection with all serological tests. Based on our findings Vaccine Manufactured with the Local Strains & Montanide ISA 71 proved to be a good Vaccine as the groups received it as booster after priming with live vaccination, showed high and good protection against IBV especially after challenge with wild live IBV virus where the virus couldn’t be detected in trachea & kidney after 7 days post challenge even by using the sensitive rRT-PCR.
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