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Comparative Transcriptome Profiling of Backfat in Anqingliubai and Yorkshire Pigs

Comparative Transcriptome Profiling of Backfat in Anqingliubai and Yorkshire Pigs

Hong Hu1, 2, Li Qian1, Yuanlang Wang1, Chaodong Wu1, Xiaodong Zhang1, Yueyun Ding 1*, Li Wang1, Xudong Wu1, Wei Zhang1, Dengtao Li1, Jian Ding1, Min Yang1 and Zongjun Yin1*

1Anhui Provincial Laboratory of Local Animal Genetic Resource Conservation and 
Bio-Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China
2College of Animal Science, Anhui Science and Technology University, Fengyang, Anhui 233100, People’s Republic of China
 
Hong Hu and Li Qian contributed equally to this work.

*      Corresponding author: dingyueyun@ahau.edu.cn; yinzongjun@ahau.edu.cn

ABSTRACT

The present work aimed to explore the molecular mechanisms and candidate genes associated with fat metabolism in Anqingliubai (obese) and Yorkshire (lean) pigs. The transcriptome profiling of backfat between Anqingliubai and Yorkshire pigs was carried out by RNA-sequencing technology. The sum of clean reads were 288.3 and 365.3 million which was obtained from the RNA sequencing data in the Anqingliubai and Yorkshire pigs, respectively. Most reads were located in exonic region, while less reads were located in intergenic and intronic regions. There were 2601 upregulated genes, but 284 downregulated genes in Yorkshire pigs compared with those in Anqingliubai pigs. The top 10 most significant Gene Ontology (GO) terms included catalytic activity, binding, cell, cytoplasm, positive regulation of multicellular organismal process, biological regulation, cellular process, etc. There were 54 significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including cytokine-cytokine receptor interaction, biosynthesis of unsaturated fatty acids, fatty acid metabolism, regulation of lipolysis in adipocytes, glycerolipid metabolism, etc. The results of differentially expressed genes from sequence were highly reliable by qRT-PCR confirmation. The present work will help understanding of the different mechanisms involved in fat deposition between lean and obese pigs.
 

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Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

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